Biochemical and proteomic analyses reveal that Populus cathayana males and females have different metabolic activities under chilling stress.
Identifieur interne : 002B84 ( Main/Exploration ); précédent : 002B83; suivant : 002B85Biochemical and proteomic analyses reveal that Populus cathayana males and females have different metabolic activities under chilling stress.
Auteurs : Sheng Zhang [République populaire de Chine] ; Lihua Feng ; Hao Jiang ; Wujun Ma ; Helena Korpelainen ; Chunyang LiSource :
- Journal of proteome research [ 1535-3907 ] ; 2012.
Descripteurs français
- KwdFr :
- Acides aminés (métabolisme), Activation enzymatique (MeSH), Azote (métabolisme), Basse température (MeSH), Feuilles de plante (métabolisme), Glutamate-ammonia ligase (analyse), Glutamate-ammonia ligase (métabolisme), Glutathion (analyse), Glutathion (métabolisme), Métabolisme énergétique (MeSH), Nitrate reductase (analyse), Nitrate reductase (métabolisme), Photosynthèse (MeSH), Populus (métabolisme), Populus (physiologie), Protéines végétales (analyse), Protéines végétales (métabolisme), Protéome (analyse), Protéome (métabolisme), Protéomique (méthodes), Spécificité d'espèce (MeSH), Stress physiologique (MeSH).
- MESH :
- analyse : Glutamate-ammonia ligase, Glutathion, Nitrate reductase, Protéines végétales, Protéome.
- métabolisme : Acides aminés, Azote, Feuilles de plante, Glutamate-ammonia ligase, Glutathion, Nitrate reductase, Populus, Protéines végétales, Protéome.
- méthodes : Protéomique.
- physiologie : Populus.
- Activation enzymatique, Basse température, Métabolisme énergétique, Photosynthèse, Spécificité d'espèce, Stress physiologique.
English descriptors
- KwdEn :
- Amino Acids (metabolism), Cold Temperature (MeSH), Energy Metabolism (MeSH), Enzyme Activation (MeSH), Glutamate-Ammonia Ligase (analysis), Glutamate-Ammonia Ligase (metabolism), Glutathione (analysis), Glutathione (metabolism), Nitrate Reductase (analysis), Nitrate Reductase (metabolism), Nitrogen (metabolism), Photosynthesis (MeSH), Plant Leaves (metabolism), Plant Proteins (analysis), Plant Proteins (metabolism), Populus (metabolism), Populus (physiology), Proteome (analysis), Proteome (metabolism), Proteomics (methods), Species Specificity (MeSH), Stress, Physiological (MeSH).
- MESH :
- chemical , analysis : Glutamate-Ammonia Ligase, Glutathione, Nitrate Reductase, Plant Proteins, Proteome.
- chemical , metabolism : Amino Acids, Glutamate-Ammonia Ligase, Glutathione, Nitrate Reductase, Nitrogen, Plant Proteins, Proteome.
- metabolism : Plant Leaves, Populus.
- methods : Proteomics.
- physiology : Populus.
- Cold Temperature, Energy Metabolism, Enzyme Activation, Photosynthesis, Species Specificity, Stress, Physiological.
Abstract
Male and female poplars (Populus cathayana Rehd.) respond differently to environmental stresses. However, little is known about sex-dependent responses to chilling at the proteome level. To better understand these differences, a comparative proteomics investigation combined with a biochemical approach was used in the current study. Three-month-old poplar cuttings were treated at 25 or 4 °C for 14 days. Results revealed significant sexual differences in nitrogen metabolic enzymes and free amino acid components in response to chilling. The chilling-treated males showed higher activities of nitrate reductase and glutamine synthetase and higher contents of reduced glutathione, serine, arginine, leucine, glycine, proline and methionine than chilling-treated females. A total of 65 chilling-responsive spots were found, of which 48 showed significant sexual differences. These proteins are involved in photosynthesis, carbon and energy metabolism, metabolic processes of proteins, lipid metabolism, vitamin metabolism, stress defense, and gene expression regulation. The study shows that males have more effective metabolic processes and protective systems to chilling than females.
DOI: 10.1021/pr3005953
PubMed: 23072643
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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last="Feng">Lihua Feng</name></author><author><name sortKey="Jiang, Hao" sort="Jiang, Hao" uniqKey="Jiang H" first="Hao" last="Jiang">Hao Jiang</name></author><author><name sortKey="Ma, Wujun" sort="Ma, Wujun" uniqKey="Ma W" first="Wujun" last="Ma">Wujun Ma</name></author><author><name sortKey="Korpelainen, Helena" sort="Korpelainen, Helena" uniqKey="Korpelainen H" first="Helena" last="Korpelainen">Helena Korpelainen</name></author><author><name sortKey="Li, Chunyang" sort="Li, Chunyang" uniqKey="Li C" first="Chunyang" last="Li">Chunyang Li</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:23072643</idno><idno type="pmid">23072643</idno><idno type="doi">10.1021/pr3005953</idno><idno type="wicri:Area/Main/Corpus">002849</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002849</idno><idno type="wicri:Area/Main/Curation">002849</idno><idno 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(MeSH)</term><term>Feuilles de plante (métabolisme)</term><term>Glutamate-ammonia ligase (analyse)</term><term>Glutamate-ammonia ligase (métabolisme)</term><term>Glutathion (analyse)</term><term>Glutathion (métabolisme)</term><term>Métabolisme énergétique (MeSH)</term><term>Nitrate reductase (analyse)</term><term>Nitrate reductase (métabolisme)</term><term>Photosynthèse (MeSH)</term><term>Populus (métabolisme)</term><term>Populus (physiologie)</term><term>Protéines végétales (analyse)</term><term>Protéines végétales (métabolisme)</term><term>Protéome (analyse)</term><term>Protéome (métabolisme)</term><term>Protéomique (méthodes)</term><term>Spécificité d'espèce (MeSH)</term><term>Stress physiologique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Glutamate-Ammonia Ligase</term><term>Glutathione</term><term>Nitrate Reductase</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" 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However, little is known about sex-dependent responses to chilling at the proteome level. To better understand these differences, a comparative proteomics investigation combined with a biochemical approach was used in the current study. Three-month-old poplar cuttings were treated at 25 or 4 °C for 14 days. Results revealed significant sexual differences in nitrogen metabolic enzymes and free amino acid components in response to chilling. The chilling-treated males showed higher activities of nitrate reductase and glutamine synthetase and higher contents of reduced glutathione, serine, arginine, leucine, glycine, proline and methionine than chilling-treated females. A total of 65 chilling-responsive spots were found, of which 48 showed significant sexual differences. These proteins are involved in photosynthesis, carbon and energy metabolism, metabolic processes of proteins, lipid metabolism, vitamin metabolism, stress defense, and gene expression regulation. The study shows that males have more effective metabolic processes and protective systems to chilling than females.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23072643</PMID><DateCompleted><Year>2013</Year><Month>05</Month><Day>23</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1535-3907</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>12</Issue><PubDate><Year>2012</Year><Month>Dec</Month><Day>07</Day></PubDate></JournalIssue><Title>Journal of proteome research</Title><ISOAbbreviation>J Proteome Res</ISOAbbreviation></Journal><ArticleTitle>Biochemical and proteomic analyses reveal that Populus cathayana males and females have different metabolic activities under chilling stress.</ArticleTitle><Pagination><MedlinePgn>5815-26</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/pr3005953</ELocationID><Abstract><AbstractText>Male and female poplars (Populus cathayana Rehd.) respond differently to environmental stresses. However, little is known about sex-dependent responses to chilling at the proteome level. To better understand these differences, a comparative proteomics investigation combined with a biochemical approach was used in the current study. Three-month-old poplar cuttings were treated at 25 or 4 °C for 14 days. Results revealed significant sexual differences in nitrogen metabolic enzymes and free amino acid components in response to chilling. The chilling-treated males showed higher activities of nitrate reductase and glutamine synthetase and higher contents of reduced glutathione, serine, arginine, leucine, glycine, proline and methionine than chilling-treated females. A total of 65 chilling-responsive spots were found, of which 48 showed significant sexual differences. These proteins are involved in photosynthesis, carbon and energy metabolism, metabolic processes of proteins, lipid metabolism, vitamin metabolism, stress defense, and gene expression regulation. The study shows that males have more effective metabolic processes and protective systems to chilling than females.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Sheng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Lihua</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Hao</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Wujun</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Korpelainen</LastName><ForeName>Helena</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chunyang</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>10</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Proteome Res</MedlineTA><NlmUniqueID>101128775</NlmUniqueID><ISSNLinking>1535-3893</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000596">Amino Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 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IdType="doi">10.1021/pr3005953</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Feng, Lihua" sort="Feng, Lihua" uniqKey="Feng L" first="Lihua" last="Feng">Lihua Feng</name><name sortKey="Jiang, Hao" sort="Jiang, Hao" uniqKey="Jiang H" first="Hao" last="Jiang">Hao Jiang</name><name sortKey="Korpelainen, Helena" sort="Korpelainen, Helena" uniqKey="Korpelainen H" first="Helena" last="Korpelainen">Helena Korpelainen</name><name sortKey="Li, Chunyang" sort="Li, Chunyang" uniqKey="Li C" first="Chunyang" last="Li">Chunyang Li</name><name sortKey="Ma, Wujun" sort="Ma, Wujun" uniqKey="Ma W" first="Wujun" last="Ma">Wujun Ma</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Sheng" sort="Zhang, Sheng" uniqKey="Zhang S" first="Sheng" last="Zhang">Sheng Zhang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce 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